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Core Store Ordering Information Contact the Core Store Vendor Contact Information Downloads Shop Now Research Services DNARNA Processing Bisulfite Conversion cDNA Synthesis DNA Isolation FFPE NA Isolation Gene Expression Arrays MicroRNA profiling Nucleic Acid Quality Assessments Real Time PCR Equipment RNA Isolation RNAseq Sample Plating Whole Genome Amplification Sequencing High Throughput Sequencing Long Read Sequencing Medium Throughput Sequencing PCR Support Pyrosequencing Sanger Sequencing Whole Exome/Targeted Sequencing Whole Genome Sequencing Single Cell Genomics Bioprocessing Services Overview Blood Processing SARS-CoV-2 Cell Culture Clinical Trial Support Cell Line Authentication DNA Services Mycoplasma Detection RNA Isolation Johns Hopkins BioBank Storage Services Bioshipping Shipping Services Cell Line Distribution Dry Ice Genotyping Custom Genotyping Genome Wide Association Non-human Genotyping Targeted Content Products Cell Line Testing Cell Line Authentication Mycoplasma Detection Digital PCR Methylation Microbiome Products Cell Biology Products Core Store Dry Ice Synthetic Biology Products Assays (qPCR, dPCR, SNP, CNV) CRISPR Genes and Gene Fragments Oligonucleotides Oligo Library Synthesis (OLS) RNA Interference (RNAi, si/shRNA) Ordering Johns Hopkins Customers External Customers PromotionsGRCF Career/Job Opportunities GRCF Faculty and Staff Directory Grant Resources Hours, LocationsContact Acknowledgements Join the GRCF E-Mail List Publications Useful Links Weather PolicyAlerts Upcoming Events 4/25/24 – 2024 GRCF Core Symposium Core Store Ordering Information Contact the Core Store Vendor Contact Information Downloads Shop Now Research Services DNARNA Processing Bisulfite Conversion cDNA Synthesis DNA Isolation FFPE NA Isolation Gene Expression Arrays MicroRNA profiling Nucleic Acid Quality Assessments Real Time PCR Equipment RNA Isolation RNAseq Sample Plating Whole Genome Amplification Sequencing High Throughput Sequencing Long Read Sequencing Medium Throughput Sequencing PCR Support Pyrosequencing Sanger Sequencing Whole Exome/Targeted Sequencing Whole Genome Sequencing Single Cell Genomics Bioprocessing Services Overview Blood Processing SARS-CoV-2 Cell Culture Clinical Trial Support Cell Line Authentication DNA Services Mycoplasma Detection RNA Isolation Johns Hopkins BioBank Storage Services Bioshipping Shipping Services Cell Line Distribution Dry Ice Genotyping Custom Genotyping Genome Wide Association Non-human Genotyping Targeted Content Products Cell Line Testing Cell Line Authentication Mycoplasma Detection Digital PCR Methylation Microbiome Products Cell Biology Products Core Store Dry Ice Synthetic Biology Products Assays (qPCR, dPCR, SNP, CNV) CRISPR Genes and Gene Fragments Oligonucleotides Oligo Library Synthesis (OLS) RNA Interference (RNAi, si/shRNA) Ordering Johns Hopkins Customers External Customers PromotionsGRCF Career/Job Opportunities GRCF Faculty and Staff Directory Grant Resources Hours, LocationsContact Acknowledgements Join the GRCF E-Mail List Publications Useful Links Weather PolicyAlerts Upcoming Events 4/25/24 – 2024 GRCF Core Symposium © 2022 Genetic Resources Core Facility  High quality, cost-efficient research services and products for the Johns Hopkins scientific community and beyond. VIEW - This Week at the GRCF -April 22, 2024 Core Store Biobank Clinical Blood Cellular Processing DNA/RNA Processing Sequencing Covid-19 Processing genotyping Synthetic Biology Products Dry Ice A Few Things That Make the GRCF Great CORE STORE This is a non-profit resource that offers appreciable savings and fast delivery of a wide variety of research products with no shipping and handling fees and free Core Store delivery to three Johns Hopkins campuses. read more Johns hopkins Biobank This College of American Pathologist (CAP) accredited facility is dedicated to meeting all of your short and long-term cryostorage requirements for basic science and clinical research needs. read more PROCESSING SERVICES With more than 30 years of expertise, our facilities offer leading edge service support for processing of biomaterials, cells, nucleic acids and derivatives for both basic science and clinical research needs. read more HIGH THROUGHPUT SEQUENCING Our goal is to provide the research community with access to ‘next generation’ sequencing platforms as well as foundational sequencing alternatives. read more The GRCF has been a part of the Johns Hopkins Scientific Community for more than 30 years! Cell Line Authentication As recommended by NIH prior to publication , we offer short tandem repeat (STR) profiling service to to confirm the identity of a human cell line. Mycoplasma Testing Testing service is recommended prior to cryopreservation and at regular intervals in culture, using PCR based MycoDtect kit from Greiner Bio-One . Single Cell Genomics We offer a growing range of genomics applications for single cells, including RNA-seq, gene expression profiling by qPCR and DNA amplification by way of the 10x Genomics Chromium platform . Lab Service Offerings The GRCF is a JHU service center producing a number of products and services to aid researchers performing studies in cell biology, molecular biology and genetics. Request Services Genotyping We offer high throughput options on the llumina platform, medium throughput with the Life Technologies Open Array, and lower throughput options via individual TaqMan® , Qiagen Pyrosequencing assays or STR/VNTR analysis. Digital PCR Digital PCR is a new service offering to address nucleic acid detection and quantification, by measuring the fraction of negative replicates to determine absolute copies. Methylation DNA methylation service is offered for large numbers of samples via genotyping using the Illumina 850K Methylation BeadChip or via custom Illumina products. Smaller scale projects and quantitative analysis can be carried out via Pyrosequencing. Ordering From the GRCF is Easy! Core Store 500,000 + Products Volume Pricing Fast, Free, On-Campus Delivery Shop Now Johns Hopkins Service Customers iLab Based Requests GeneSifter Requests Direct Billing Through SAP Order Services Now External Service Customers Service Offerings are Available to Customers Outside the Johns Hopkins Community Invoicing, Direct PayPO Options Available ORDER SERVICES NOW Study Design HelpLetters of Support We encourage investigators planning a study to meet with us to discuss objectives. We have a broad range of expertise and in many instances, can propose an array of possible approaches to your research question. We are also happy to write letters of support for grant applications, demonstrating to reviewers that the resources for your study are available at the university. ResearchEnvironment Document Leading Edge Technologies The GRCF works to stay at the leading edge of technology, by providing sophisticated tools and equipment oftentimes not available in the individual research lab. We encourage faculty to reach out and discuss their evolving research needs. Service Overview Educational Opportunities The GRCF offers regular webinars and seminars to the Johns Hopkins Community. Annually, the GRCF Symposium combines guest research speakers and vendors offering the latest technology, for a unique progressive research experience. Events Testimonials For the past two decades, the GRCF has provided state-of-the-art technical expertise and cost-efficient support for our laboratory’s collaborations with scientists working at Johns Hopkins and throughout the world.” Bert Vogelstein, M.D. Director, Ludwig Center at Johns Hopkins Howard Hughes Medical Institute We have been very pleased with the affordable, high-quality deep RNA sequencing data we have received from the GRCF for multiple research projects over the last year. I now consider it an essential feature of our projects.” Gregg Semenza, M.D., Ph.D....